FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937262
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41340.21339395497356578No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30620.15805812533358937No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.13064830673393688No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.10514840016476862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG250.007790337351.011121
TACGTAG250.007790337351.011121
AGCGTCA1800.047.2239883
CAGCGTC2000.042.5015872
GTATCAA73550.042.0757941
GTGGTAT17950.040.9699361
TGGTATC19700.037.755222
GGTATCA35950.036.3013761
TATCAAC87650.035.8341982
ACGGAGT601.5955829E-435.4179882
ATCAACG91150.034.5514833
TCAACGC93300.033.8008354
GCGTCAG2650.033.6805044
CAACGCA94100.033.6038065
TATACGT1157.4196578E-933.262114
AACGCAG99300.031.8012896
CGTCAGA3100.028.7913975
GTACACG600.00679947528.3395121
TACGGAC755.905833E-428.3343933
CGCAGAG112000.028.0427328
Produced by FastQC (version 0.11.5)