FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937262
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94440.48749214097009075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21850.038.665391
TGGTATC23500.035.8159072
GTATCAA92050.029.3278791
GTGCTAG1650.028.3530941
GTGCGAG507.033673E-427.511
ATCGAAC904.7255526E-827.49857938
AGAGTAC47300.025.63953411
CGGCTGG1301.0004442E-1025.38592720
TATCAAC107300.025.0960462
TCGGCTA1151.9123036E-823.91242843
TGCGTAC1052.1000596E-723.57142812
CCGAACG701.8015593E-423.570824
CGTACGT1052.1010965E-723.57021114
ATCAACG113350.023.3149533
CAGAGTA52450.023.27931410
ACTCCGA952.3183893E-623.15669828
TCAACGC114050.023.1471444
CAACGCA115400.022.9478475
GTATTAC1800.022.9326481
TCGCTGC600.002036335922.9160756
Produced by FastQC (version 0.11.5)