FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018418
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95100.3150657066052482No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA36090.11956594480949956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1150.038.2606524
TGGTATC24850.034.980632
GTGGTAT25900.033.5797461
GTACGTG758.021743E-932.9987214
CAATGCG1700.029.11748119
GTATCAA104200.028.1565571
GTACTAG2650.025.9647541
TATCAAC116100.024.8547952
AGAGTAC53250.024.16807411
ATCGTCA2100.023.57051310
TCGGTAA600.002036752222.91577515
CAGAGTA56450.022.84676610
ATCAACG125800.022.6691633
CAACGCA126400.022.5169265
TCGTCCG1605.638867E-1122.34288440
TCAACGC129150.022.1013474
ACGGGTA1757.2759576E-1221.99914616
TACGGGT4050.021.7275515
GAGTACG58350.021.67867912
AAGCCGT2800.021.6066637
Produced by FastQC (version 0.11.5)