FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872352
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46220.5298319944242691No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.3970874142547962No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT30450.349056344228018No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.270418363229522No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA20160.231099372730274No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT19610.22479457833535088No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.163007593265104No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG13680.15681743149554309No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACG13400.1536077179854004No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT13040.14948094347235977No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1477614540919262No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA12720.14581269946076814No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC12070.13836157881222258No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT11550.13240068229338617No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT11540.13228604966802393No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.12907633615788122No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC11230.12873243828179448No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA11190.12827390778034556No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA11010.12621052052382525No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC11010.12621052052382525No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10940.12540809214628956No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.12059352188107553No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA10490.12024962400498881No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.11726917574557061No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA10000.11463262536223909No Hit
GAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGG9550.10947415722093834No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG9460.10844246359267817No Hit
GGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTT9330.10695223946296907No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG9150.10488885220644878No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC9100.10431568907963759No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.10133524082021936No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGA8730.10007428194123472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1000.055.251753
GTGCCTA250.007792883651.004549
GCCTAAG250.007794649351.0016172
TATACGT2050.045.6112024
TACGTGC2000.044.6289756
ATACGTG2100.044.525225
GTATCAA85950.040.062811
ATTCAAG1001.886292E-938.2599871
TGGTATC23700.038.1974142
GTGGTAT23350.038.0506131
TATCAAC96650.035.839212
AGACGTT955.1310053E-835.792666
CAGTACA858.6906766E-735.0031179
GTACTGG751.4806288E-534.0088731
CGTGCAA2600.032.693348
GGTATCA44400.032.553541
TATTCTC2900.032.24245
GTACAGC1850.032.170561
CTAGAGG802.3060653E-531.876014
ATCAACG109950.031.6972313
Produced by FastQC (version 0.11.5)