FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094528
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67780.21903178772336201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19700.034.9307941
TGGTATC20900.032.9049762
GTATCAA79300.027.2824921
TATCAAC88900.024.2285732
GGTATCA38500.023.3072781
ATCAACG94650.022.2554113
AGAGTAC46050.022.0956411
CAACGCA95250.021.9990255
TCAACGC96650.021.5950074
AACGCAG106500.020.0889596
CAGAGTA52800.019.73966410
AGTACGG52200.019.22900813
CGGACGT1301.6379345E-619.03792436
GAGTACG55200.018.13413212
CGCAGAG116750.018.0431928
ACGCAGA119600.017.5899547
TACGGGG31450.017.31186915
GTACGGG58400.017.0928414
GCAGAGT124650.017.0540969
TACATCG2355.329639E-1016.38225441
Produced by FastQC (version 0.11.5)