FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054486
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.18473455313773723No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15850.15031019852326158No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.13959407711434765No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA12080.11455818284927444No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.10128157225415985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2850.037.280143
GTATCAA58400.032.90271
GTCGACT4250.031.0083561
AACGCAG64800.029.7116096
TATACGT1452.1827873E-929.311294
TATCAAC67400.028.8793872
ATCAACG69700.027.8044593
TCAACGC71100.027.2582664
CAACGCA71800.026.9333255
CGACTTT4750.026.8417023
CGCAGAG72000.026.4453018
GCGTCAG4300.025.6985054
ACGCAGA74750.025.5861137
GCAGAGT75700.025.2088749
TCGACTT5300.024.8581162
TACCGCG1353.1725885E-522.037758
TTAAACG3000.021.95904522-23
CGTCAGA5100.021.6673685
GTGGTAT24250.021.5625111
GCAGCGT4550.021.4893071
Produced by FastQC (version 0.11.5)