FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054486
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32830.31133651845543703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG453.7878632E-430.5658342
TTAAACG2250.029.3334622
GTGGTAT24650.025.0160641
GTATCAA69550.023.511331
TGGTATC26250.023.3697622
AGAGTAC32550.023.31696711
CAGAGTA33550.023.11375610
AACGCAG74850.021.7124926
TATACGT1658.367351E-1121.6657334
CGCTCGA903.995093E-521.38897922
TATCAAC77800.021.250722
ATACGTG1701.2551027E-1021.0285055
TACGTGC2001.8189894E-1220.6241116
ATCAACG80250.020.3904633
TCAACGC81050.020.1533574
CAACGCA82200.019.8379525
AGTACGG38250.019.69849613
GAGTACG38200.019.65229612
AGTCGCG1401.5305523E-719.64201216
GGTACGG856.681077E-419.412773
Produced by FastQC (version 0.11.5)