FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044
Sequences flagged as poor quality0
Sequence length91
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTGAGC1081.3426156141223273No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGTGGCGG811.0069617105917454No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGGTGGCGG480.5967180507210343No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGTGGGGG450.5594231725509696No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGATGGCGG420.522128294380905No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT280.34808552958727No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTGAGCGA280.34808552958727No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGTTGCGG270.33565390353058183No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACAC260.32322227747389354No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGGCGG220.27349577324714075No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGG210.2610641471904525No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGGGGGGG200.24863252113376427No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTGTGGCGG190.2362008950770761No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGCCGT180.22376926902038788No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGTTGGCGG170.21133764296369967No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGATGGCGG170.21133764296369967No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGTTGGCGG170.21133764296369967No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGGGGCGG160.19890601690701143No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGTGTGGCGG160.19890601690701143No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACACTG160.19890601690701143No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGATGCCGG150.18647439085032322No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGGTGGGG140.174042764793635No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGGATGGCGG140.174042764793635No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGGTGGGGG130.16161113873694677No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGGTTGCGG130.16161113873694677No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGTTGGGG130.16161113873694677No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGATGCCGT120.14917951268025859No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGAGGGGGGGTGGCGG120.14917951268025859No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGTTGGGGG110.13674788662357037No Hit
GCCGGTGGAGTGGCGGCATGGACGAGCTGTACAAGGGAGGGGGAGGTCCT110.13674788662357037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGGTTGGCGG110.13674788662357037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGGCGT110.13674788662357037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT110.13674788662357037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTTTGGCGG110.13674788662357037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGGGGGGCGG100.12431626056688214No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGATTGCGG100.12431626056688214No Hit
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACA100.12431626056688214No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGGATGGCGG100.12431626056688214No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGGCGG100.12431626056688214No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGCCGG90.11188463451019394No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGTTTGGGG90.11188463451019394No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGGGTGGCGGGGT90.11188463451019394No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGGCGT90.11188463451019394No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGGGTGTTGCGG90.11188463451019394No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACACTGAGCGAT90.11188463451019394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC4700.055.1595731
TTATACA4850.054.32992
TATACAC5100.051.6666683
TACACAT5650.046.637175
ATACACA5600.046.294644
ACACATC6000.043.9166646
CACATCT6150.042.8455287
TCGGATG255.2139058E-542.540-41
ACATCTC6200.042.58
GCGTATG255.2139058E-542.540-41
CATCTCC6150.042.1544729
GGTGGGA404.9820846E-737.18750456-57
CGGATGG358.691455E-636.4285740-41
GCGTGGA301.5264149E-435.41666856-57
ATGCCGG301.5264149E-435.41666844-45
GTCGGAT301.5264149E-435.41666838-39
GCGGATG250.002707991534.040-41
GTGGGAA507.253402E-834.058-59
TGGGCGT250.002707991534.052-53
GGATGGC402.1692376E-531.87500242-43
Produced by FastQC (version 0.11.5)