FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192226
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.20029759458357727No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.1552557988166673No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.140241866894364No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.10560078374402168No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA250.007786290751.0166131
GTATCAA59150.038.23731
GTGGTAT16000.037.996751
CCTATAC1500.036.8345153
TGGTATC16850.035.8171732
CGACTTT5400.035.41783
GGTATCA32150.033.3234521
TATCAAC68700.033.035992
TATACGT1456.184564E-1132.2424164
ATCAACG73050.031.0105783
GTACTAG1101.8568971E-730.919161
CAACGCA75050.030.2974415
TCAACGC74650.030.2320484
ATACGTG1701.2732926E-1130.0009635
GTCGACT6950.029.362081
AACGCAG81550.027.8316126
CTACGCT408.614675E-426.56223760-61
CGCAGAG85800.026.1546868
GACAGGT3600.025.9806841
TGCTTAA3300.025.75732218-19
Produced by FastQC (version 0.11.5)