FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650195
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25090.1520426373852787No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.12659109983971592No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.12228857801653743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18850.040.8099982
GTGGTAT18950.040.152161
GTATCAA65550.039.297511
TATCAAC76400.034.0453152
GGTATCA34250.033.013091
ATCAACG82200.032.0050243
TCAACGC84000.031.4203934
CAACGCA84350.031.3917455
AACGCAG91250.028.8782776
GTCGACT6600.026.4061471
CGCAGAG101200.025.786198
ACGCAGA104750.025.1557337
GCAGAGT108950.024.1079659
CGACTTT7350.022.551563
TATACGT3250.022.23134
ATACGTG3450.020.9431635
CAGAGTA54900.020.9021410-11
TCGAGGG23400.020.887259
GTCGAGG44700.020.8226458
GCGTCAG3300.020.6065524
Produced by FastQC (version 0.11.5)