FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650195
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60920.36916849220849657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19200.036.403131
TGGTATC18800.036.2807542
GCGTTAT350.004100889431.4274113
TTAAACG3050.028.85314422
GTATCAA76900.028.1256431
AGAGTAC45600.024.96618511
TATCAAC88400.024.2674882
CAGAGTA50450.023.11114110
GGTATCA35600.022.7249851
ATCAACG95100.022.1502193
CGAACTA1254.6284185E-821.99918729
AGTACGG51100.021.84850913
TCAACGC97450.021.6436354
CAACGCA97900.021.6003275
GAGTACG52650.021.51867312
ACGGGAT2950.021.43988616
AACGCAG102050.020.7488656
GCGTGCC956.0633734E-520.2624113
TACGGGG29500.020.22806715
GTACGGG55400.020.00377514
Produced by FastQC (version 0.11.5)