FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292728
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34410.2661812848487849No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15910.12307306718814787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA458.848632E-636.6644979
GTGGTAT30850.035.0547521
TGGTATC31050.034.9872442
CTCCGAC350.004100231531.4279328
GTATCAA112400.028.154061
CGATAAT400.00787427627.49943728
TACCGAG507.0348254E-427.4983777
CTATCGG904.7233698E-827.49837312
ATCGGTC904.7233698E-827.49837314
AGAGTAC56000.026.31987411
TATCGGT957.9780875E-826.05109413
TATCAAC127400.024.7610032
CAGAGTA60500.024.58945510
GAGTACG61350.023.5764412
AGTACGG61350.023.39715213
ATCAACG136750.022.9840183
GGGCGTC852.5718164E-522.645727
AACGCAG139050.022.6038426
CAACGCA139400.022.4090065
TCAACGC139900.022.3485724
Produced by FastQC (version 0.11.5)