FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508417
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58700.036.133961
GTGGTAT14400.036.0122641
TATCAAC61700.034.8537252
ATCAACG63200.033.690273
CAACGCA63700.033.4278225
TGGTATC16250.032.1688542
TCAACGC66950.031.9314444
GTCGACT5500.030.9136581
CGCAGAG76700.027.59758
AGCGTCA6550.027.251593
AACGCAG80200.027.0820986
GAACCGT1106.4948326E-627.0483726
GGTATCA32150.026.4424741
CGTCAGA7050.025.9232315
GCAGAGT84900.024.8819549
ACGCAGA91950.023.4826857
GCGTCAG7750.023.0329064
GACAGGT5850.020.3448851
TCGAGGG20450.019.537569
GTACTAG6150.019.3524531
Produced by FastQC (version 0.11.5)