FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34119
Sequences flagged as poor quality0
Sequence length91
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.7884170110495619No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.6594566077552098No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.37222661859960726No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.3663647820862276No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1190.3487792725460887No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCCTAAGA1090.3194700899791905No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT920.26964447961546356No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT900.2637826431020839No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.21395703273835695No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.20516427796828746No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGGGGGGTGGCGG690.20223335971159764No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA680.1993024414549078No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGGCGGGTGGCGG680.1993024414549078No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.18757876842814855No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.18757876842814855No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.17878601365807908No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA590.17292417714469943No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA560.16413142237462996No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA560.16413142237462996No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.1553386676045605No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA490.14361499457780122No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.1377531580644216No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT440.12896040329435213No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG430.1260294850376623No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGGGGGGTGGGGG420.12309856678097249No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC380.1113748937542132No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.10258213898414373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATG150.001015427485.1160054
GCAAGAT150.001015427485.1160053
CGCAAGA150.001015427485.1160052
CCGCAAG200.00317944463.8370061
AAGATGG250.007690245851.0696035
TGGTATC1200.046.1045042
GTGGTAT1250.044.2603231
GGCGTTT209.262481E-442.495638-39
GCGTGGA209.262481E-442.495656-57
CGTGGAA209.262481E-442.495656-57
GTGTGCC209.262481E-442.495642-43
TGTGCCG209.262481E-442.495642-43
ACTTTTT655.354159E-639.284311
CGTATAA450.001629528737.8293341
GAGATGG903.002242E-837.8293341
GTATCAA4100.037.3680041
GCGTGGT359.12776E-636.424846-47
GATGTGT601.5508047E-435.4650041
ATGTGTA601.5508047E-435.4650042
TGTGTAT601.5508047E-435.4650043
Produced by FastQC (version 0.11.5)