FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076010
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.16163796606643022No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA45750.14873163611301654No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.12178113855286557No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC31840.10351071680521196No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90400.041.8044171
GTGGTAT21100.039.084821
GGTATCA44450.036.150051
TGGTATC23850.034.3932382
TATCAAC111550.033.985952
ATCAACG113300.033.6860853
TCAACGC116550.032.710814
CAACGCA120000.031.734965
GCGTCAG5300.031.2751874
AGCGTCA5350.030.9823913
CGCAGAG142600.026.5866498
AACGCAG148300.026.1961466
GCAGAGT151400.025.1255389
GTCGACT9200.024.027271
ACGCAGA166450.023.262317
AGAGTAC62600.022.20179710-11
CGTCAGA7950.021.3847455
TCGAGGG33800.021.2514369
CAGAGTA70400.021.2512610-11
TACCGAG1909.583691E-720.1329387
Produced by FastQC (version 0.11.5)