FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540663
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.2742974940009593No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32290.20958509420944102No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.19160582164951062No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.14935128577761653No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT20000.12981424230996655No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA19530.12676360761568234No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA17380.11280857656736094No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA16940.10995266323654167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21900.049.118241
TGGTATC22750.046.7072222
GTATCAA90650.042.024891
TATCAAC108800.035.8233222
GGTATCA47600.033.942441
ATCAACG118350.032.6812443
CAACGCA120350.032.139185
TCAACGC121450.031.8830874
GCGTCAG4700.031.652624
ATACGTG3250.031.388276
TATACGT3300.029.624665
AGCGTCA4950.029.1943723
AACGCAG133650.028.8772896
TAGACCG1652.8012437E-1028.3366325
TCAGATG5550.028.3338747
ACCGTCC1203.9904444E-728.3338748
CGACTTT8100.027.810983
CGCAGAG145300.026.4423488
GCAGAGT150350.025.6107319
ACGCAGA155400.024.7511167
Produced by FastQC (version 0.11.5)