FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568891
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82000.5226621862194378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAC358.642329E-539.3063961
GTGGTAT18100.039.0675161
TGGTATC17850.038.5246282
CGACGAG1000.035.7505824
CAATGCG1100.035.00168219
AACGACG501.8206116E-532.9994829
GTATCAA77050.030.2105751
TGCGACG1350.028.51989222
CCGGTTC606.317143E-527.50132454
TTATCGG507.032405E-427.50044648
ACGACGA507.0337154E-427.49956930
TAGGTAG1900.027.4986934
GGCCTAC400.007875822527.4986927
TATCAAC88500.026.1079432
AGAGTAC41950.025.43383411
ATACGAT651.0890914E-425.38502747
AATCCGT550.001226410625.00120221
CAGAGTA44700.024.17670410
TTGCGTG1151.9123036E-823.9119057
CGCCGAA1151.9123036E-823.91190527
Produced by FastQC (version 0.11.5)