FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247173
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT4000.040.3828431
GTATCAA45150.035.117561
AGGCGTC500.002789024534.001319
GGTATCA19750.033.5761451
ATCAACG46150.033.5209273
TGGTATC10900.033.1420022
TATCAAC47200.032.955312
CAACGCA47350.032.7611545
TCAACGC49300.031.3791224
GTACGCC1003.0952124E-629.7511469
GCGTCAG5750.029.5650394
GTGGTAT13000.029.4287931
TACCGAG600.006805668628.3344238
CGCAGAG58650.026.4502938
CGACTTT5900.025.9320473
AACGCAG63650.025.0402416
TATAGTG4650.024.677275
GCAGAGT64650.024.061259
TGCGACG1152.1827873E-1124.02159522-23
CGTCAGA7650.023.8887465
Produced by FastQC (version 0.11.5)