FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727147
Sequences flagged as poor quality0
Sequence length91
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.19432109325899716No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA12820.17630547880964922No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12490.1717671942537066No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC12030.16544110063027145No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA11480.1578772930370338No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.15691462661607625No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10280.14137444010633338No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC9900.13614853667827825No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.12720949134081555No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT8360.11496987541721275No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC8330.11455730409394524No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC8240.1133195901241427No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA8000.11001901953800264No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT7960.10946892444031263No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT7850.1079561629216651No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.10630587762859504No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA7690.10575578253090502No Hit
ATATTATACCATGTCTATTTAGTGACATTCACAAAAACATCTTTGAGTTA7410.10190511684707496No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC7370.10135502174938493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA900.061.3959433
CGACTTT3450.048.0493
TCGACTT3450.048.0492
GCAGCGT1250.047.6120151
TGCCGTC255.4534183E-542.49903584-85
ACAATGC655.5547116E-639.2352759
GTCGACT4350.039.0903321
GTATCAA32150.038.874511
GGTATCA13850.037.4462741
TATCAAC33600.037.3182752
TGGTATC6500.037.2735142
GCGTCAG1600.037.1943324
CGAGGCA250.00278812834.00273510-11
GGGTACC250.002789538433.9992318-19
ATACCAT2350.032.561416
CAACGCA39500.031.4256535
ATCAACG40200.030.9799273
TCAACGC40850.030.3850174
GTGGTAT7850.030.3261241
GATGTTC853.4830904E-530.0075746
Produced by FastQC (version 0.11.5)