FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531581
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117430.46386033075773603No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA25640.10128058316127353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA1150.040.65080323
CGACGAG1250.039.59866324
CGAGTCC1350.038.702427
TGCGACG1400.037.3201722
TCTACCG458.848048E-636.66687847
ACGAGTC1450.034.1367826
GTGGTAT28500.033.989951
ATCGTCA1850.032.701610
TGGTATC28550.032.6605222
ACGACGT701.6106605E-731.42751326
CTACCGG553.4928198E-529.99957848
CGGGCTA553.4932906E-529.99898717
GTCCTAC1850.029.729930
ACGGGTG653.2085463E-629.61438616
CAATGCG1850.028.24228919
GTATCAA109350.027.6335221
ACGGAAT705.711987E-627.4990738
GGGCTAG606.3221756E-527.49907118
CCGGTAC400.00787619127.49907151
GCACGAC759.761037E-625.665812
Produced by FastQC (version 0.11.5)