FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339611
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181675.349355586244261No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC150.005778455.00412432
GCACGAC150.005781768454.9960253
GAGCTTA257.851434E-443.9968249
GTGGTAT13050.037.0909041
AGTAGAC300.001923007136.66941513
ATCTGGT300.001923007136.6694158
ATCGACG300.001924398136.6640173
TGGTATC13450.036.1869772
ACCGCGA350.00409410331.43092712
ATCATAT350.00409410331.4309271
ACTTGAT453.7883045E-430.55784425
TTAAACG453.7883045E-430.55784422
CGGGGCG453.7915757E-430.55334917
GTATCAA58350.029.788011
CAGAGTA23650.028.49050710
AGAGTAC23400.028.44230311
AGCGCGC507.018576E-427.5020628
CGCGTAG507.018576E-427.50206212
GACCGCG400.00786257927.5020611
GCCCTGC400.00786257927.5020623
Produced by FastQC (version 0.11.5)