FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101508
Sequences flagged as poor quality0
Sequence length91
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.8669267446900737No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA8370.8245655514836269No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.6275367458722465No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.6186704496197344No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.5201560468140443No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA5000.49257201402845097No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGGGG4700.4630176931867439No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGGGG4420.4354336604011506No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4290.42262678803641096No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA4150.4088347716436143No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA3660.36056271426882613No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT3460.3408598337076881No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTGGCGG3440.33888954565157425No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA2790.27485518382787566No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA2790.27485518382787566No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.2551523032667376No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.23938999881782716No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC2380.23446427867754266No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.22559798242503057No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA2290.22559798242503057No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2170.2137762540883477No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA2010.19801394963943728No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.1960436615833235No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.18619222130275445No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.18619222130275445No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA1610.1586081885171612No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT1590.1566379004610474No Hit
GCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCC1530.150727036292706No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.14974189226464907No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.14777160420853527No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGATCAG1420.13989045198408007No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGGGCGG1410.13890530795602318No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA1370.13496473184379557No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA1320.13003901170351106No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.12609843559128345No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGTTGGCGG1260.12412814753516965No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.12215785947905584No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.12018757142294205No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.11920242739488512No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGTTGGGG1170.11526185128265752No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA1130.11132127517042992No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1110.10935098711431612No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAAAAAAAAAAAAAA1090.10738069905820231No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.10541041100208852No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1024549789179178No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1024549789179178No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCGGGGGGTGGGG1030.1014698348898609No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA259.1123686E-568.03
CTAAATG200.003215019163.753
GTTCAGA200.003215019163.751
CGACTCT200.003215019163.756
TCGACTC200.003215019163.755
CATCAAT200.003215019163.751
AGCGCCG1150.044.3478242
CAGCGTC450.001655912537.777782
GTATCAA15850.036.7350161
CTGGTTT301.5909121E-435.41666838-39
GTGTGCG301.5909121E-435.41666852-53
TGGTATC4350.035.1724132
TCTGGTT250.00278360734.038-39
GTGGTAT4650.032.9032251
TATACCT652.537077E-432.6923074
GCGTCAG652.537077E-432.6923071
GAGCGCC1605.456968E-1231.8751
CGACTTT2000.031.8753
TCGACTT2050.031.097562
GGTATCA8500.031.01
Produced by FastQC (version 0.11.5)