FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659924
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99251.503961062182918No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16050.2432098241615701No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.1375916014571375No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAA7710.11683163515798789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG504.1212115E-738.49973719
TCTACGC300.001925810836.6636354
TGGTATC28250.034.857242
GTGGTAT28350.034.7553631
TGCTTAA2000.032.99977519
CTGCTAC701.6072227E-731.42835418
AGTTACG350.00409851931.42835419
CGACGGG453.7939643E-430.55534610
CTGCTTA2250.030.55534418
GTATCTA553.4689736E-530.0248181
CACTGCT3400.028.30648416
GTATCAA109200.028.026831
AGAGTAC54150.027.6267711
ATATGAT400.007871008527.49981153
CTAGTGT705.70241E-627.49981145
CGTGTAC705.70241E-627.4998118
TTAAACG2100.027.49981122
CAGAGTA54800.027.49981110
GAGCGTC400.00787391927.49772828
ACCACTG4100.026.82908414
Produced by FastQC (version 0.11.5)