FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628429
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25950.15935604192752645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG3100.027.49898722
GTGGTAT27400.025.919471
GTATCAA82750.024.616181
AGAGTAC43550.023.55251911
TGGTATC29750.023.486782
TATCAAC92800.021.8768672
CAGAGTA47550.021.86039410
AACGCAG93450.021.687276
ATCAACG97700.020.6613273
TCAACGC98600.020.4429594
CAACGCA100300.020.1787195
AGTACGG52550.019.361813
GAGTACG53700.019.10078612
CGCAGAG107250.018.845468
ACGCAGA107600.018.7586027
TCTAACG750.00739901318.3394152
TATACGT1503.1648597E-718.3326574
GCAGAGT112200.018.1120769
TAAACGC4750.017.94670923
GGTATCA45250.017.459041
Produced by FastQC (version 0.11.5)