FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651082
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36010.21809940390604465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13400.039.023911
TGGTATC14000.037.329962
TAGGGTC701.6098056E-731.4281375
GTATCAA53000.029.0799831
GTCCGTT507.031307E-427.50128654
TAGTCGC507.032552E-427.50045221
TTCGACG400.00787580427.49878928
ACGGGAT957.982089E-826.05069716
TATCAAC60400.025.3203542
TTAAACG2200.025.00041422
AGAGTAC29150.024.33781611
ATCAACG63250.023.826483
TACGACC701.8012551E-423.5711044
CAACGCA64050.023.2276255
CGGAGTC600.002035708622.91704648
CGTCCGT600.002036067222.91635153
TCAACGC64950.022.7787484
CAGAGTA31850.022.2746410
AGTACGG31050.022.2286213
GGTATCA23850.022.1387313
Produced by FastQC (version 0.11.5)