FastQCFastQC Report
Fri 10 May 2024
EGAF00005411482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18343
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length91
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT2621.4283377855312653No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCT1270.6923622090170637No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGCGG1210.6596521833942103No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA1160.6323938287084991No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG1110.605135474022788No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA930.5070053971542278No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA790.4306820040342365No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT750.40887532028566753No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGGGG740.40342364934852537No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGG700.3816169655999564No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGGTGGCGG650.3543586109142452No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA600.32710025622853406No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.28348688873139616No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT520.28348688873139616No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGG490.26713187591996945No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA470.25622853404568496No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGATGGCGG470.25622853404568496No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTGTGGCGG460.25077686310854275No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGG450.24532519217140056No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.23442185029711607No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA430.23442185029711607No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA430.23442185029711607No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGTTGGCGG430.23442185029711607No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAA420.22897017935997382No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2235185084228316No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTGTGGCGG410.2235185084228316No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGG360.19626015373712044No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT350.1908084827999782No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGT350.1908084827999782No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.18535681186283595No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA340.18535681186283595No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGATGCCGG340.18535681186283595No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCA320.1744534699885515No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGTTGGCGG310.16900179905140927No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTGCAGCTAAT310.16900179905140927No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTG300.16355012811426703No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTGTGGCGG300.16355012811426703No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTTGCGG290.15809845717712478No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA280.15264678623998254No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGGCGG280.15264678623998254No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGCCGT270.14719511530284032No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC270.14719511530284032No Hit
GCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCAT260.14174344436569808No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTTTGGCGG260.14174344436569808No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGGCGT260.14174344436569808No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGGGTGGCGG250.13629177342855586No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGTATGGCGG250.13629177342855586No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTTTGGCGG250.13629177342855586No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGGGGCGG250.13629177342855586No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTTGGGG230.12538843155427137No Hit
TGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAA230.12538843155427137No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGGCGT230.12538843155427137No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGGATGGCGG230.12538843155427137No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTGGTGTGGCGG220.11993676061712916No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.11993676061712916No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGT220.11993676061712916No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGG220.11993676061712916No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGGCGT210.11448508967998691No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTATGGCGG210.11448508967998691No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGTGTGGGGG200.10903341874284468No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGGTGCCGG200.10903341874284468No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGCGGGGG200.10903341874284468No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG190.10358174780570244No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTGTGCCGG190.10358174780570244No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG190.10358174780570244No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGGGGGGG190.10358174780570244No Hit
GATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAA190.10358174780570244No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGATGCCGT190.10358174780570244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)