FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18343
Sequences flagged as poor quality0
Sequence length61
%GC76

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG853346.51910810663468No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA970.5288120809027967No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAA300.16355012811426703No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAA260.14174344436569808No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230.12538843155427137No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11993676061712916No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAA220.11993676061712916No Hit
GGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210.11448508967998691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210.11448508967998691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG200.10903341874284468No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA190.10358174780570244No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257.453632E-444.2373621
GTATCAA506.5582345E-427.6483521
CGAGGTT701.7093473E-423.56908616
GGATTGG1003.1523268E-622.1186811
TCGAGGT752.72782E-421.99781415
ATTGGGG1003.3075958E-621.9978123
GATTGGG1003.3075958E-621.9978122
TGGGGTT1003.3075958E-621.9978125
TTGGGGT1003.3075958E-621.9978124
AGGTTTT650.003122813321.15174518
GAGGTTT650.003122813321.15174517
GGGTTGT1107.554683E-619.9980147
TATCAAC700.00479757319.6409052
GGTTGTT1151.1093005E-519.1285348
TTGTTGA1151.1093005E-519.12853410
GTTGTTG1201.6013522E-518.3315129
TGTTGAT1303.188033E-516.92139611
CCAACAG1303.188033E-516.92139618
GATCCAA1303.188033E-516.92139615
GTTGATC1354.406832E-516.29467612
Produced by FastQC (version 0.11.5)