FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468204
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.17170638412645653No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.1301590242227919No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.10706958978452587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56650.041.2061351
GTGGTAT14000.035.2305451
ATCAACG69050.034.9004673
TATCAAC69450.034.2098732
CAACGCA71600.033.7180145
CGACTTT4850.033.3007283
TCAACGC73200.032.864884
TGGTATC15900.032.0771682
AACGCAG76850.031.5804926
GGTATCA29650.031.5491331
TAAGACG5550.029.101634
TAGACGT905.1530296E-528.3357985
ACGCAGA91050.026.4200487
CGCAGAG91750.026.218488
GCAGAGT95300.025.2864159
GGTGCGA850.001226582325.0004718
TCGAGGG23200.023.8151049
GTCGAGG43200.023.4147938
ACTCGCG552.0171626E-423.18146584-85
AGAGTCG53100.022.9728075
Produced by FastQC (version 0.11.5)