FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66359
Sequences flagged as poor quality0
Sequence length91
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.7338868879880649No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA4220.6359348392832924No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.6057957473741317No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT3020.4551002878283277No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.40386383158275446No Hit
ATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAA2480.3737247396735936No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.3360508747871427No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA1920.2893352823279435No Hit
AACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA1900.2863213731370274No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1780.2682379179915309No Hit
CAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAA1530.23056405310507996No Hit
GTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.2245362347232478No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT1470.22152232553233173No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.21850841634141563No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGG1420.2139875525550415No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGGGGGTGGCGG1370.20645277957775132No Hit
GATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAA1300.19590409740954506No Hit
GGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAA1130.17028586928675837No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.16576500550038428No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.16425805090492623No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.15973718711855212No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTGCAGCT990.14918850495034586No Hit
GAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCA940.14165373197305564No Hit
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC890.13411895899576545No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGG890.13411895899576545No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.12658418601847526No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG840.12658418601847526No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.12357027682755918No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGGCGT810.12206332223210115No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT800.12055636763664311No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.11904941304118506No Hit
GATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAA740.11151464006389487No Hit
ATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAA720.1085007308729788No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGG700.10548682168206272No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG700.10548682168206272No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT680.10247291249114664No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGGGTGGCGG680.10247291249114664No Hit
GGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10096595789568862No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10096595789568862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCC401.9186882E-763.7975121
CGGGTCG200.003210219663.7494356
ACGGGTC200.003210219663.7494355
TGGTATC1400.060.7137452
GTGGTAT1550.054.879581
GCGTCAG250.00774124951.0380061
AGCGCCG509.0108006E-750.9995462
CGTCAGA250.007764379550.9995462
CGCGAGA1500.042.4996262
TCGACTT801.1019438E-842.4996222
GGTATCA3500.038.8861051
GTATCAA6150.036.6533131
CCGCGAG1750.036.4557231
TCGGGTA359.187263E-636.42824638-39
CGAGATG1401.8189894E-1236.4282464
TGGGCTT359.187263E-636.42824652-53
GTCTGCG301.5880293E-435.4163552-53
TTGCCGG301.5880293E-435.4163544-45
CGACTTT1104.66207E-934.7724153
GCGAGAT1501.8189894E-1233.9997023
Produced by FastQC (version 0.11.5)