FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007201
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25940.12923469049686603No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.10327814703161267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59850.038.5642661
TGGTATC16400.036.0236632
TATCAAC66400.035.2055852
ATCAACG74400.031.4771613
GGTATCA30200.031.386821
CAACGCA74500.031.263765
GTGGTAT19150.030.186971
TCAACGC77650.030.1049694
CGACTTT7100.029.9315053
GTCGACT7700.029.8093451
AACGCAG85550.027.2269576
CGCAGAG89200.025.8250148
AGTACCG1658.6711225E-925.7592355
ACGCAGA96300.024.0092587
GCAGAGT96950.023.8044479
GTACCGT1452.0774605E-623.4509566
TCGAGGG21100.022.9629949
ATACGTG2655.456968E-1222.4542795
ATCGTAC1152.7234724E-422.1753433
GTACTAG3550.020.3549461
Produced by FastQC (version 0.11.5)