FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007201
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96830.48241307173521736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17100.033.1331142
GTGGTAT17950.032.500481
GTATCAA71650.029.1119581
CGGTCGA400.00787508627.4995910
TATCAAC78350.026.4330392
GGCGTCG651.0894706E-425.3842358
GCGGTAT550.00122217825.0164531
AGAGTAC42000.024.94605611
ATCAACG84650.024.2672083
GGTATCA32050.023.6114711
CAACGCA86650.023.6112925
CAGAGTA45900.023.18592810
TCAACGC88200.023.1651764
CGCACCG600.002036233222.91632515
AACGCAG91400.022.6856586
AGTACGG47750.021.59653713
ACCGTGT1559.0221874E-1021.2900038
CGGCCCG650.003240863621.1535335
CGATCGT650.003240863621.1535326
CCCCGAA6200.020.8464612
Produced by FastQC (version 0.11.5)