FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117563
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.17735018070569622No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.1352049056742215No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA12920.11560869499079693No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.11202947842761438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10150.043.1430661
GTATCAA45350.041.905411
TATACGT709.245816E-636.429824
TGGTATC12150.036.379852
TATCAAC53750.035.1081772
CAACGCA57850.032.768365
TCAACGC58300.032.441084
ATCAACG58350.032.4132773
GGTATCA25000.030.6106341
GGACCTA703.9351505E-430.3676911
ATACGTG853.4888784E-530.0023675
AACGCAG67500.028.2096356
ACGATCG705.032598E-827.3211460-61
CGCAGAG70800.026.5934268
GCAGAGT74150.025.5066039
AGCGTCA2700.025.1860473
CGACTTT4800.024.7925173
ACGCAGA77250.024.5380557
GTCGACT5100.024.1750661
GTACTAG2002.5975169E-923.3831211
Produced by FastQC (version 0.11.5)