FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274853
Sequences flagged as poor quality0
Sequence length61
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA21520.78296398438438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11060.4023969176250578No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8220.2990689568605764No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.24121985206637728No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTT5130.18664522490203855No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTT5110.18591756320651404No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.17536646862140853No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.13643656791084688No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.1327982594332243No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.13097910519441303No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3580.1302514434988885No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.12879612010783947No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.11096840856748881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG202.610292E-454.99926840
TTAAACG2650.046.69749522
GCTTAAA2800.046.16010320
CTTAAAC2750.045.9993921
TGCTTAA3300.044.1660819
CTGCTTA3400.042.8670818
CACTGCT3550.042.6050716
ACTGCTT3500.041.64230317
AGACTAC358.651185E-539.28519411
ACCACTG4050.038.0241914
TACCACT4250.036.88186613
AGTCCTA458.820518E-636.6661829
CTACGGG300.001923203436.6661814
CCACTGC4350.036.03400815
GACAGGT558.6318687E-735.0186461
TAAACGC3550.034.85869223
ATACCAC5300.033.20710812
GAGTCCT501.8151497E-532.99956528
ATCGTCA501.8151497E-532.99956510
CAATGCG501.8151497E-532.99956519
Produced by FastQC (version 0.11.5)