FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130954
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.2451242421002795No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.19014310368526352No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC1890.14432548833941689No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.13287108450295523No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC1710.1305802037356629No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA1670.1275256960459398No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA1630.12447118835621669No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.12294393451135514No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA1490.11378041144218581No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC1410.1076713960627396No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA1340.10232600760572415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC259.074614E-568.071862
GCGTCAG302.236123E-456.726564
CGTCAGA302.236123E-456.726565
GTACTGG401.435529E-553.221831
CAGATGT354.8133655E-448.5670628
ACGGGCA452.8942637E-547.272135
AGCGTCA409.2409283E-442.5449143
GACTTCG801.1057637E-842.5449146
TAGATCA409.293244E-442.496188
GTATCAA5950.040.0729141
ACTTCGC851.9073923E-839.9964077
TTCGCTC851.9073923E-839.9964079
AGATCAG450.001658402537.7743849
ATAGATC450.001658402537.7743847
GACGTGG601.5886711E-435.4134839
TATCAAC6950.034.8929522
GGTTAGG751.4554216E-534.0619741
GACCAGA1553.6379788E-1232.96321
GTGGTAT1850.032.2207871
TCAACGC7550.032.1200034
Produced by FastQC (version 0.11.5)