FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130954
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8890.678864334040961No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA1360.1038532614505857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTG150.005772532454.99923725
CGGGGAA750.043.9993917
ACGGGGA1250.037.39948316
TCAGAAT300.001919537736.66615712
AGAATCT300.001919537736.66615742
GTGGTAT1900.033.301731
CAATCCA350.004086780431.42813554
CCAAAAG350.004086780431.42813530
CAAAGTG350.004086780431.42813529
AGGTTAC453.7776848E-430.5551327
GTCAATA553.458813E-530.0110421
GTATCAA7650.029.4879741
GTGTATA852.6611815E-829.1283651
CGAGGGG952.4538167E-928.94696810
AGAGTAC4200.028.15437111
TACGGGG2850.027.98206715
TGTATAA904.6362402E-827.5101222
TCTTCAC400.00784864127.49961915
CCAATCC400.00784864127.49961953
ATCCAAT400.00784864127.49961951
Produced by FastQC (version 0.11.5)