FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29212
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATATACCAGGCAAAAGGCACAGAACAAATTATTTGTTCACAGTTACT970.33205531973161717No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGGGGGTGGCGG880.30124606326167325No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGGGGGTGGGGG870.29782281254279064No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.26701355607284677No Hit
TCCATGGACTGCACATAGTCCTCAAAAGCAGTGATCTGCTCCTCCAGCAT770.2635903053539641No Hit
GTTATATACCAGGCAAAATGGACACACCATCAGGAGATAAGACCTGTATC680.23278104888402026No Hit
GTATGATACCTAAAAAGAAAAAAGAGTTAATCACCTCTCCTGGATATGAA650.2225112967273723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.20539504313295906No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.20197179241407642No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGGGGGGGGGGG570.19512529097631112No Hit
ATATGAATCAAAGAAAGCCAAAAAACCTGCACTTGTTGCCAAGTCTTCCA560.19170204025742846No Hit
GTATCATACCGTTCCACTGCCACCAAAAGGAAGGGTTTTAATCCATTGGA560.19170204025742846No Hit
CTACATTATAGATCTAGAAAGGCAATTATCAATTTAGGAGAAGTCAACAA560.19170204025742846No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGT520.17800903738189786No Hit
GTCTATGATATATTTGAATCAAAATGTGGTAGTTTGGTATTTCAGATAGC510.1745857866630152No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1745857866630152No Hit
ATAATGTAGAGAGCAAAAAAGTTAAAAGTTAGCTATCTGAAATACCAAAC500.17116253594413255No Hit
CTTTGGATCTGATGATGAGGAGGAAAGTGAAGAAGCAAAGAGGCTAAGGG490.1677392852252499No Hit
GACTAGAGGAATCGCCATTAATTTCATTTGTGTTTGACAAAGCGTCATCC470.1608927837874846No Hit
CTTCAAATACAGTGTGTAGTTGAAGATGATAAAGTTGGAACAGATATGCT440.15062303163083662No Hit
GTCTTCCATCTTACTAGATGTGAAACCTTGGGATGATGAGACAGATATGG420.14377653019307135No Hit
ATCCAGGATGGATTTTAGATCTTGTTGAAAGCAGCCACATCCATGGACTG410.14035327947418869No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT410.14035327947418869No Hit
GTATTTGAAGTTTCTTAATTCCGTATCCCACTGGAACTAGTTTAGATGAG410.14035327947418869No Hit
GTTGCTAACTTAGCAAAATGAAGTTTCTATAAAGAGGACTCAGGCATTGC410.14035327947418869No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.13693002875530602No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.13350677803642338No Hit
ATGGAAGACTTGGCAACAAGTGCAGGTTTTTTGGCTTTCTTTGATTCATA390.13350677803642338No Hit
ACTACACACTGTATTTGAAGTTTCTTAATTCCGTATCCCACTGGAACTAG380.13008352731754075No Hit
ACATTATAGATCTAGAAAGGCAATTATCAATTTAGGAGAAGTCAACAAAC380.13008352731754075No Hit
ATTTAGGAGAAGTCAACAAACTTCTAATAGCATTCATATCCAGGAGAGGT370.12666027659865808No Hit
CTACAGGAAGTGGAGCTACAGATAGTAAAGATGATGATGACATTGACCTC370.12666027659865808No Hit
ATGTAGCTCTTGTCCGCCAGGTAATCGTTGAGCACCTGGAGGCCGGCAGG360.12323702587977543No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.12323702587977543No Hit
CTTCTAATAGCATTCATATCCAGGAGAGGTGATTAACTCTTTTTTCTTTT350.11981377516089278No Hit
GTATTTGAAGAGACACTTGAGAAAACTATACATGAAGAGTAACCAAAAAA350.11981377516089278No Hit
GGTATAATCACATCAAGTCTTACGAAAAGGAAAAGGCCAGCCTGCCAGGA340.11639052444201013No Hit
CATGAAGAGTAACCAAAAAAATGAATGGTTGCTAACTTAGCAAAATGAAG340.11639052444201013No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGGGTGGCGG340.11639052444201013No Hit
CTCTTTCTGCTGCTCCCCAGCTCTCGGATACAGCCGACACCATGGGTTTC340.11639052444201013No Hit
GGCTAAGGGAAGAACGTCTTGCACAATATGAATCAAAGAAAGCCAAAAAA330.1129672737231275No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGGGGGGTGGGG330.1129672737231275No Hit
ATGTGAAACCTTGGGATGATGAGACAGATATGGCGAAATTAGAGGAGTGC330.1129672737231275No Hit
CTTCAGCGTGGGGCGCCCACAATTTGCGCGCTCTCTTTCTGCTGCTCCCC320.10954402300424482No Hit
CTATAAAGAGGACTCAGGCATTGCTGAAAGAGTTAAAAGTAACTGTGAAC320.10954402300424482No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10612077228536218No Hit
CCTCTAATTTCGCCATATCTGTCTCATCATCCCAAGGTTTCACATCTAGT310.10612077228536218No Hit
CTCTTCATGTATAGTTTTCTCAAGTGTCTCTTCAAATACTGCATAATGGT310.10612077228536218No Hit
GTTAGCAACCATTCATTTTTTTGGTTACTCTTCATGTATAGTTTTCTCAA310.10612077228536218No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.10269752156647953No Hit
ATCCAGGAGAGGTGATTAACTCTTTTTTCTTTTTAGGTATCATACCGTTC300.10269752156647953No Hit
GATTATACCAACGTAGGGCATGACACAAGTCGGCAGGCGGTGGGCTGGAC300.10269752156647953No Hit
GTATCAACGCAGAGTACGGGGCGGCTTTGGGAAACCGAATGTCCGTTGCC300.10269752156647953No Hit
CAACTACACACTGTATTTGAAGTTTCTTAATTCCGTATCCCACTGGAACT300.10269752156647953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC555.456968E-1269.545452
GTGGTAT555.456968E-1269.545451
GTATCAA2500.035.6999971
GGTATCA1800.035.4166681
GAGAAAG250.00276747134.062-63
GCGTGGA250.00276747134.056-57
ATGTCGT250.00276747134.036-37
ATACCTA500.002744924334.06
ATCGCGG250.00276747134.036-37
TACCTAA500.002744924334.07
CAAACCC250.00276747134.082-83
TATCAAC2850.032.807022
TTATTGA550.004378084630.909094
ATCGGGG353.8980276E-430.35714536-37
TCATCGG353.8980276E-430.35714534-35
CATCGGG353.8980276E-430.35714536-37
CAACGCA3400.028.755
TCAACGC3300.028.3333364
ATCAACG3300.028.3333363
ATGATAC600.0066991328.3333323
Produced by FastQC (version 0.11.5)