EGAF00005411499 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411499
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29212
Sequences flagged as poor quality	0
Sequence length	61
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4598	15.74010680542243	No Hit
GCTATATACCAGGCAAAAGGCACAGAACAAATTATTTGTTCACAGTTACTTTTAACTCTTT	92	0.3149390661372039	No Hit
GTATCATACCGTTCCACTGCCACCAAAAGGAAGGGTTTTAATCCATTGGATGACGCTTTGT	79	0.27043680679172943	No Hit
GTATGATACCTAAAAAGAAAAAAGAGTTAATCACCTCTCCTGGATATGAATGCTATTAGAA	59	0.20197179241407642	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.1882787895385458	No Hit
CTACATTATAGATCTAGAAAGGCAATTATCAATTTAGGAGAAGTCAACAAACTTCTAATAG	51	0.1745857866630152	No Hit
GTATTTGAAGAGACACTTGAGAAAACTATACATGAAGAGTAACCAAAAAAATGAATGGTTG	46	0.15746953306860195	No Hit
ATAATGTAGAGAGCAAAAAAGTTAAAAGTTAGCTATCTGAAATACCAAACTACCACATTTT	44	0.15062303163083662	No Hit
TCCATGGACTGCACATAGTCCTCAAAAGCAGTGATCTGCTCCTCCAGCATATCTGTTCCAA	40	0.13693002875530602	No Hit
GTTATATACCAGGCAAAATGGACACACCATCAGGAGATAAGACCTGTATCTTACGTGTAAG	38	0.13008352731754075	No Hit
CTTTGGATCTGATGATGAGGAGGAAAGTGAAGAAGCAAAGAGGCTAAGGGAAGAACGTCTT	38	0.13008352731754075	No Hit
GTTGCTAACTTAGCAAAATGAAGTTTCTATAAAGAGGACTCAGGCATTGCTGAAAGAGTTA	37	0.12666027659865808	No Hit
CTTCAAATACAGTGTGTAGTTGAAGATGATAAAGTTGGAACAGATATGCTGGAGGAGCAGA	35	0.11981377516089278	No Hit
ATTTAGGAGAAGTCAACAAACTTCTAATAGCATTCATATCCAGGAGAGGTGATTAACTCTT	35	0.11981377516089278	No Hit
GTCTTCCATCTTACTAGATGTGAAACCTTGGGATGATGAGACAGATATGGCGAAATTAGAG	33	0.1129672737231275	No Hit
CTACAGGAAGTGGAGCTACAGATAGTAAAGATGATGATGACATTGACCTCTTTGGATCTGA	32	0.10954402300424482	No Hit
ATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.10612077228536218	No Hit
ATATGAATCAAAGAAAGCCAAAAAACCTGCACTTGTTGCCAAGTCTTCCATCTTACTAGAT	31	0.10612077228536218	No Hit
GACTAGAGGAATCGCCATTAATTTCATTTGTGTTTGACAAAGCGTCATCCAATGGATTAAA	31	0.10612077228536218	No Hit
ATGTAGCTCTTGTCCGCCAGGTAATCGTTGAGCACCTGGAGGCCGGCAGGGCTTTTCAGGT	30	0.10269752156647953	No Hit
ACTACACACTGTATTTGAAGTTTCTTAATTCCGTATCCCACTGGAACTAGTTTAGATGAGC	30	0.10269752156647953	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCA	15	0.005727527	55.0	22
CGCCATA	15	0.005727527	55.0	24
CCATATC	15	0.005727527	55.0	26
AATACTG	15	0.005727527	55.0	7
ATACTGC	15	0.005727527	55.0	8
CGGGAAA	30	0.001895091	36.666668	17
ACGGGAA	30	0.001895091	36.666668	16
GTGGTAT	50	1.7603046E-5	33.0	1
TTGTTCA	35	0.004035129	31.428572	36
TTCACAG	35	0.004035129	31.428572	39
GTTCACA	35	0.004035129	31.428572	38
GCTATAT	35	0.004035129	31.428572	1
TCACAGT	35	0.004035129	31.428572	40
AGAGTAC	115	1.8189894E-11	28.69565	11
CAGAGTA	125	1.8189894E-12	28.6	10
GTATCAA	265	0.0	28.01887	1
TACGGGA	50	6.8680575E-4	27.500002	15
CAGTTAC	40	0.007750206	27.499998	43
GGCACAG	40	0.007750206	27.499998	19
TTACTTT	40	0.007750206	27.499998	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content