FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806475
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.18073873150749387No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.1365089469823828No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.11043607024730483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG302.6446596E-670.851881
GTATCAA61850.040.552251
GTGGTAT16000.040.1198771
TGGTATC17800.036.2946052
TATCAAC71050.036.1319472
TACGGAC500.002788475734.0023164
CGACTTT7300.033.187193
ATCAACG79150.032.3805963
CAACGCA81000.031.9034045
GTCGACT8350.031.0560361
TCAACGC82700.031.0420174
AACGCAG91200.028.430836
TTGTACG905.1543437E-528.335264
GGTATCA34050.027.5916581
CGCAGAG100500.025.7977758
CCTATAC5850.024.7025383
ACGCAGA105750.024.4768437
GCAGAGT108200.023.9226079
AGCGTCA3850.023.1833973
CCCTATA4950.023.1833952
Produced by FastQC (version 0.11.5)