FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954127
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.15157663754709905No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.11304280632732672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58450.039.0546151
GGTATCA27050.038.973281
TATCAAC65650.034.7642752
GCGTCAG4050.034.630894
AGCGTCA4200.033.393223
ATCAACG70300.032.525243
TCAACGC73050.031.1852574
CAACGCA73600.031.06855
TGGTATC15950.030.3765072
GTGGTAT16200.029.6514531
AACGCAG88100.026.2452076
CGTCAGA5450.025.735545
CGCAGAG90450.025.0934098
TCGAGGG21550.023.4707349
GCAGAGT98900.022.9924099
ACGCAGA100800.022.8120177
AATACCG950.002342844822.3698085
CGGTCCA1152.7230108E-422.1758569
TAGACTG5200.022.0686765
GTCGACT7000.021.8618551
Produced by FastQC (version 0.11.5)