FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173978
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20921.2024508845946038No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA17661.0150708710296705No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA17360.997827311499155No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.9581671245789698No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.9047120900343721No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT11530.6627274712894734No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.6247916403223396No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.4805205255836945No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAA8110.46615089264159837No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.3759095977652347No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA6280.3609651795054547No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6120.35176861442251317No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5910.3396981227511524No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.3276276310797917No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5620.32302934853832094No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5450.31325799813769556No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5420.311533642184644No Hit
ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4740.27244824058214256No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.26612560208762026No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.2655508167699364No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.2529055397808918No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.24830725723942107No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.23911069215647954No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4080.2345124096150088No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4010.23048891239122188No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.22359148857901573No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAA3870.22244191794364804No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA3560.20462357309544882No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA3310.19025394015335273No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.17875823379967581No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAAAAAAAAAAAAA3070.17645909252894046No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA2990.17186080998746966No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2840.163239030222212No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2790.16036510363379278No Hit
GTGGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTT2720.15634160641000586No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.15231810918621894No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.15116853855085124No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.14714504132706435No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.14197197346790974No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.13047626711423282No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT2190.12587798457276209No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAA2150.1235788433020267No Hit
GTATCAACGCAGAGTACGGGGAGCTTTTGGCCCGGGTTATACCTGCTTGC2140.12300405798434284No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTT2120.12185448734897517No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2010.11553184885445286No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGGTTTTTTTTTT2010.11553184885445286No Hit
ATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAA1980.11380749290140132No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1940.11150835163066596No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1850.10633528377151134No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10633528377151134No Hit
GTATCAACGCAGAGTACGGGCACGTATAAGACGAGCAAAAAAAAAAAAAA1830.10518571313614365No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1810.10403614250077595No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1760.10116221591235675No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1740.10001264527698904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCA150.001025762685.048321
AGCGTCA302.653178E-884.999433
ATATCAA650.065.421781
CAGATCA200.003211641263.786241
CAGCGTC401.94721E-763.749572
TTTACAT200.003218970563.749574
GCTGCTC200.003218970563.749578
CGCTGCT200.003218970563.749577
TTATCAA803.6379788E-1253.1551971
CTGCTCT250.007785409750.9996539
TATTTAC250.007785409750.9996532
TACATTA250.007785409750.9996536
TCAGAGT250.007785409750.9996539
TTACGCT250.007785409750.9996534
TTACATT250.007785409750.9996535
CACTGCA209.310746E-442.49971416-17
TCAGATG603.1932595E-642.4997147
TCGAGTG409.297884E-442.4997149
GCGTCAG603.1932595E-642.4997144
TGCACGC209.310746E-442.49971418-19
Produced by FastQC (version 0.11.5)