FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173978
Sequences flagged as poor quality0
Sequence length61
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51782.97623837496695No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA18341.0541562726321718No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16840.9679384749795952No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5670.32590327512674017No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.28969180011265794No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.28739265884192255No Hit
GGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.24026026279184726No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.22876455643817034No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.20979664095460343No Hit
ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.20634792904850036No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.20060007587166195No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAA3040.17473473657588892No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA2860.1643886008575797No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.155766821092322No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2660.15289289450390278No Hit
GGGTTATACCTGCTTGCACGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.1488693972801159No Hit
GTATCAACGCAGAGTACGGGGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.12242927266665901No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAAAAAAAAAAAAA1980.11380749290140132No Hit
GTATCAACGCAGAGTACGGGGAGCTTTTGGCCCGGGTTATACCTGCTTGCACGTATAAGAC1910.10978399567761442No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTT1770.10173700123004058Clontech Universal Primer Mix Long (95% over 23bp)
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.10058743059467289No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)