FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638035
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.28339275256014423No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.25219528929676827No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52120.19757129833379772No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT51410.19487990113853684No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT43990.16675290509792326No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40120.15208289503361405No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.14696544966234337No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26800.10159076737041017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT48350.053.7217521
TGGTATC51350.051.3993722
GTATCAA188800.039.584241
CGACTTT15800.036.3147743
TCGACTT16100.035.63812
TATCAAC219950.034.8206982
GGTATCA94800.034.0360261
ACGTGCA2900.033.709557
GCGTCAG6150.032.482234
GTCAGAT6300.031.7106516
CAACGCA254400.030.023575
ATCAACG256900.029.9116923
GTCGACT20000.029.1203121
AACGCAG261950.029.1106456
TCAACGC269150.028.5355914
CAGTGGT8450.027.6701451
CTAACTG2003.6379788E-1227.6271744
GCATATA1405.019683E-827.328761
GTACTAG4750.026.8493081
TCGAGGG70250.026.6818169
Produced by FastQC (version 0.11.5)