FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638035
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG386541.465257284304416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT55250.043.733821
GCTACGC850.042.05895220
TGGTATC60250.039.851132
ATCGTCA2200.036.24942410
TGCTTAA4300.033.89545419
GTACTAG2650.032.1937071
GCATCGT2400.032.082828
CTGCTTA4900.030.30621518
CGTTGTG653.2048538E-629.6188432
ACCACTG7500.029.33286914
GTATCAA234100.028.9546431
CTGCTAC1251.8189894E-1228.60008818
CTTAAAC4250.028.47067621
GTATCGG1600.027.520431
CACTGCT7050.026.91395816
GCTTAAC1256.184564E-1126.4000820
TACGTGG1057.2705006E-926.1900615
CTAGACG651.0896047E-425.38421231
TATCAAC268800.025.2112772
AGAGTAC143200.025.1375211
Produced by FastQC (version 0.11.5)