FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451699
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT55560.38272396688294197No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45930.31638790134869554No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44570.30701956810606057No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT41120.28325431098319964No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37550.25866243622128277No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT31710.21843371112055596No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.1872977800494455No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25410.1750362850701144No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.16615014545026208No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG18920.13033004775783408No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.11083564843676272No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG14860.1023628176364384No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG259.151426E-568.00273
TGGTATC36900.059.3180772
GTGGTAT36350.058.8307571
AGCGTCA2400.042.501693
CGTTGTG505.4514072E-542.5016862
GTATCAA151000.041.754671
ACGTGCA1500.039.670987
CGACTTT13900.039.4440163
GCGGTAT1207.2759576E-1238.9719621
TCGACTT14400.037.7792782
CTATAAG709.227733E-636.4413151
TATCAAC178800.036.273812
ATACGTG2350.036.1728945
TCTCTGC3600.035.4205138
GGTATCA76000.034.8510061
TCGACAT1008.065399E-834.001352
GCGTCAG3200.033.2044454
TATACGT2750.032.4558334
TCATGGA1051.2380588E-732.382242
CTAGTAC2050.031.0987953
Produced by FastQC (version 0.11.5)