FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475102
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.23184837387516255No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.20046071390317416No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT25440.1724626500404718No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24650.1671070881877999No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.15517570988311316No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19010.12887244407505377No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.119381574969053No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15300.10372164094415166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23100.058.327862
GTGGTAT23950.055.2060551
GTATCAA96000.041.0525671
TATCAAC114400.035.5189932
GGTATCA49750.034.3530121
ATCAACG126700.032.2053
TCGACTT9300.031.9921822
CGTCAGA3750.031.7362445
TCAACGC129900.031.3789354
CGACTTT9550.031.1546923
AGCGTCA3550.031.1296143
CAACGCA131850.030.9470945
GCGTCAG3900.030.515624
AACGCAG141450.028.7866636
TTCGGCG556.50129E-627.04518552-53
TCGAGGG34900.025.2074859
CGCAGAG162200.025.049078
ACGCAGA165500.024.6009627
CAGCGTC4550.024.2879432
GTCGACT12850.024.1519151
Produced by FastQC (version 0.11.5)