FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936378
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261811.3520603931670367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26400.041.8045581
ACGGCTA401.9025344E-434.37343251
TGGTATC32850.033.9985662
ACGGGTC658.402094E-833.84461216
CGAGCAT453.7969364E-430.55495310
ACGTCGA507.035545E-427.49874728
GTATCAA106450.027.4058531
GTCCTAG3500.025.1631551
TATCAAC123400.023.6298312
AGAGTAC61650.023.50724211
TCCGAGC600.00203626422.9162148
CGATCGT600.00203626422.91621413
ACGGGTA1800.022.91562516
TCAAACG600.002036569622.91562327
CGAAGTA852.5718877E-522.64661422
CAGAGTA64700.022.56910710
AGCGCGG752.8740262E-421.99956714
CGGACGC752.8740262E-421.99956719
ATCAACG130950.021.7150883
TATATCG903.9980525E-521.38846848
Produced by FastQC (version 0.11.5)