FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519291
Sequences flagged as poor quality0
Sequence length91
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT28220.5434332580383638No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22300.42943166740806216No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.4199957249403513No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.4140260470526159No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.3271768622987882No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16010.3083049773633666No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT15700.3023352994756312No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.24783791746824038No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11050.2127901311596003No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG9550.18390459299313872No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8300.1598333111877541No Hit
GTATCAACGCAGAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.13922829396234482No Hit
GGTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA7220.13903572370790174No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG7110.1369174509090279No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.13152548378462173No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGG6130.11804556597360633No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.11323130961252939No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5660.10899476401478168No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA5360.10321765638148937No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5310.102254805109274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG401.9497566E-763.773551
AGTCAAC200.003220231363.7612652
CCCTTAG454.4013723E-756.6876031
CCTTAGT454.4072294E-756.6766852
TGGTATC13250.054.217132
GTGGTAT13500.053.223361
CTATCAA401.4539544E-553.1446271
TTAGTCT509.1202855E-751.0090144
CTTAGTC509.1202855E-751.0090143
TCGACAT703.4560799E-948.5800132
ACATTTT802.1100277E-1047.820955
AGTCTCC551.7591319E-646.371836
GTATCAA66800.044.106861
TCGACTT8950.043.694872
TTCGACG409.3065825E-442.507513
GTCTCCA603.2057924E-642.4993257
CACAGTT409.31541E-442.4993258
CGACTTT9200.041.5834353
TAGGCAT1650.041.2194065
GTCGACA1056.730261E-1140.4911421
Produced by FastQC (version 0.11.5)