FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519291
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258194.971971399465811No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.15136021999225868No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC5480.1055284994348063No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTAC5220.1005216728192863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAT305.4052543E-755.026011
GAGTAGT257.834223E-444.020811
TGTTATC257.852828E-443.9996152
GTGGTAT17750.042.935791
TGGTATC17950.042.5901572
TAGGCAT1050.039.285375
CACTGCT850.038.8231916
CTTAAAC504.1190106E-738.49966421
AGTCCTA1000.038.49966429
ACGAGTC1000.038.49966426
GAGTCCT1050.036.66634828
GCCATAC300.001924735536.6663442
TGCACGC300.001924735536.66634419
TTCTCTG1000.035.7496877
TACTAGG1250.035.1996882
ATTCTCT1100.034.9996956
ATCGTCA1200.034.374710
GCTCCAT401.900212E-434.374711
GTACGTG401.900212E-434.374714
TCTATAG401.900212E-434.37475
Produced by FastQC (version 0.11.5)