FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530252
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18800.35454840340064725No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.25742477161802313No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.2527100322110996No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.204431100684203No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.19066406161598637No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT9180.17312523102223093No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA8360.15766088576752185No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.1450253841569669No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.13088116593619636No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.11409669364754872No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT6010.11334233534244095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7950.057.745071
TGGTATC8250.057.1909562
AGCGTCA1150.051.74743
GTATCAA38800.046.2316131
GGTATCA17500.038.8633541
TATCAAC48400.038.554712
ATCAACG54400.034.5367663
TCGACTT4350.034.2008632
CTAGTAG751.4805624E-534.0054363
GTATGGT500.002785455434.0054321
GCTGTCG500.002786743734.0022289
TCAACGC55300.033.9746864
CAACGCA56000.033.6259085
GCAGCGT1800.033.0608371
CAGCGTC1850.032.16732
GTCTAAG802.3023547E-531.8800931
GCGTCAG1900.031.3207934
CGACTTT4900.031.2294773
AACGCAG61100.030.961226
TAGCGGC550.00444444630.9111148
Produced by FastQC (version 0.11.5)