FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530252
Sequences flagged as poor quality0
Sequence length61
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83041.5660478414037098No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA7480.14106500305515116No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC150.00578225354.9991541
CGTGTAT150.00578225354.9991510
ACGCTAT150.005784411454.9939658
ACGAACC351.5704809E-647.14212852
GTGGTAT11850.044.1004641
TGGTATC12550.041.417612
CGAACCT403.933719E-641.2532553
AGGCCGT300.00192483536.666119
CAAACGA401.899288E-434.3777149
GGTACGA350.0040979731.42808537
GGCCGTA350.0040979731.42808520
CAGGCCG350.0040979731.42808518
GCCGTAC453.7931575E-430.55508421
GTATCAA48000.028.1923831
GTGTATC400.00786272527.5047631
TGGTACG400.00786634527.50216736
TCGAGCG400.00786996627.4995759
TTCGCAG400.00786996627.49957511
CGAGCTA507.0313987E-427.49698429
GTCTTAC759.726422E-625.6711121
Produced by FastQC (version 0.11.5)